FastQCFastQC Report
Mon 6 Jun 2016
ERR1378268.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378268.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239102
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGG754.7293724E-1132.9994132
TCGTATG801.0004442E-1030.93694543
CGCCGCT300.00514583230.0018836
ACGCTGT300.00514786329.9994629
CGTATGC852.0372681E-1029.11712544
CCGATCG852.0372681E-1029.11712531
TCTCGTA952.3646862E-1128.42054441
GACCGAT903.965397E-1027.49950629
TCGGAAT1001.3460522E-924.74955635
GAGCGGT1101.5097612E-1024.5469956
GCGGTTC1052.3683242E-923.5710058
ACCGATC1052.3683242E-923.57100530
CTCGTAT1104.0545274E-922.49959842
CGGTTCA1104.0545274E-922.4995989
CGGAATC1201.1055818E-820.6246336
GATCGGA1201.1055818E-820.6246333
AGACCGA1201.1055818E-820.6246328
ATCGGAA1201.1055818E-820.6246334
AGCGGTT1251.7671482E-819.8004467
ATCTCGT1251.7680577E-819.79964640
Produced by FastQC (version 0.11.5)