FastQCFastQC Report
Mon 6 Jun 2016
ERR1378265.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378265.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524507
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAC300.00514794429.99980432
CGTATGC751.6983504E-623.99984444
CCGTCAG751.6983504E-623.99984431
CGTCAGG802.9759813E-622.49985332
AGACCGT855.0342733E-621.17633228
GACCGTC908.254619E-619.99986829
ACCGTCA908.254619E-619.99986830
ATCTCGT1051.4462912E-619.2855940
GCGGTTC1051.446746E-619.2849568
GAGCGGT1102.2556887E-618.410186
AGAGCGG1354.2718966E-818.3344155
CTCGTAT1002.047463E-517.99988242
TCGTATG1002.047463E-517.99988243
CGGAAGA1406.449045E-817.6859971
AGCGGTT1205.174039E-616.8754437
CGGTTCA1401.2562978E-616.0702699
TCTCGTA1209.756019E-514.99990241
GAGACCG1251.3809965E-414.39990527
GAGCGCT1000.0075528313.49991145
AACGGGA2501.4842954E-913.49902612
Produced by FastQC (version 0.11.5)