FastQCFastQC Report
Mon 6 Jun 2016
ERR1378261.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378261.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557531
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTT207.0308486E-444.999113
CCGGTAA356.2433555E-638.57066331
ACCGGTA401.5593441E-533.74932530
GCGGTTC401.5593441E-533.7493258
GACCGGT401.5593441E-533.74932529
TCTCGTA401.5593441E-533.74932541
GAGCGGT501.979508E-631.4993726
CGTATGC453.488127E-529.99940344
CGGTAAG554.160527E-628.63579232
ATCTCGT507.15287E-526.99946240
CTCGTAT507.15287E-526.99946242
TCGTTGG709.287487E-725.716084
CATCTCG551.3671539E-424.54496439
AGCGGTT551.3671539E-424.5449647
TCGTATG551.3671539E-424.54496443
AGAGCGG602.4641343E-422.501575
TTCGTTG802.9683997E-622.5015683
CGGAAGA654.2247848E-420.776271
CTTCGTT654.2273442E-420.7744062
CGGTTCA654.23503E-420.7688169
Produced by FastQC (version 0.11.5)