FastQCFastQC Report
Mon 6 Jun 2016
ERR1378260.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378260.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670700
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTA352.8191874E-432.1427530
CGCTAAG352.8191874E-432.1427532
CCGCTAA453.4887777E-529.999931
TCGTATG406.163565E-428.12490743
GAGCGGT450.001226045724.9999186
AGACCGC450.001226045724.99991828
TCTCGTA500.00226367822.49992441
GCGGTTC550.003934929620.4544778
GACCGCT550.003934929620.45447729
CCGGCTA807.557197E-519.68743516
GTATGCC706.979474E-419.2856545
CTCGTAT600.006507859618.74993942
AGCGGTT600.006507859618.7499397
CCGAGAC600.006507859618.74993925
GCCACCG851.20352226E-418.5293526
CCACCGC800.001709880316.8749457
CGTGAGC1257.6115157E-616.2011551
CGGCTAA850.002563727615.88230117
GCCCGGC1150.001200894413.69560614
ATCTCGG1403.6030673E-412.857100519
Produced by FastQC (version 0.11.5)