FastQCFastQC Report
Mon 6 Jun 2016
ERR1378258.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378258.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283043
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTC751.6985177E-623.9990048
GTCGATC802.9762741E-622.49906536
CGGTTCA855.0347753E-621.175599
GAAGTCG855.0347753E-621.1755933
GACCGAA754.604642E-520.99912829
CGTATGC908.255442E-619.9991744
ATGCCGA908.255442E-619.9991722
AATGCCG807.566288E-519.68668421
CGGCGTG600.00649343618.7587241
AGACCGA851.2049638E-418.5286428
AGCGGTT1002.0469817E-517.9999547
TCGTATG1002.0476682E-517.99925243
AGTCGAT1002.0476682E-517.99925235
GAGCGGT1053.1098258E-517.1448176
CTCGTAT1053.1139898E-517.14214542
TCTCGTA1053.1139898E-517.14214541
AAGTCGA1053.1139898E-517.14214534
ACCGAAG952.82057E-416.57825930
CGGAAGA1104.6197492E-516.371251
GAGACCG1156.785894E-515.65152427
Produced by FastQC (version 0.11.5)