FastQCFastQC Report
Mon 6 Jun 2016
ERR1378256.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378256.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073252
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA352.820511E-432.14235741
CTCGTAT406.166437E-428.12456342
CGTATGC550.003936724320.4542344
CCGAGAC952.8194705E-416.5786925
AGATCGC850.00256528215.88210727
CGGAAGA900.003745956615.004661
GAATCGC1251.3804997E-414.399775512
CGTGAAC950.005376260314.21030518
AACGGGT950.005376260314.21030512
AATGCCG1000.00755136213.49979121
AGAATCG1504.2706124E-513.49979111
AACCCGG1851.5276419E-613.37817122
GAACCCG2104.5062734E-712.85694321
ACCCGGG2104.5062734E-712.85694323
CCGTGTT1250.00225161312.59980311
CGGAGGT1350.004007252411.66648633
CCTTCGT1558.469451E-411.616511
CGTGAGC1953.8307637E-511.54204751
AAACGGG2801.0360782E-711.249825511
CGGCTAA1600.001107931411.249825541
Produced by FastQC (version 0.11.5)