FastQCFastQC Report
Mon 6 Jun 2016
ERR1378255.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378255.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294266
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC551.3685164E-424.5449744
GCGGTTC551.3685164E-424.544978
TCGTATG651.5241769E-524.23029143
CGGAACC602.4680715E-422.49955632
CTCGTAT602.4680715E-422.49955642
GACCGGA602.4680715E-422.49955629
AGACCGG702.7037457E-522.49955628
CGCCCGG1500.022.49868613
TCTCGTA654.2391932E-420.7688241
ACGCCCG1009.061805E-720.2488212
CCGGCTA2200.019.43068316
CGGCTAA2250.018.9988917
AGCGGTT750.001110473517.9996437
GAGACCG750.001110473517.99964327
CCTTCGT1459.6340955E-817.0745641
CGGTTCA800.001711670816.8740169
GTCCGGC850.002566398615.88142614
CCGGAAC1004.168172E-415.74968931
GCCCGGC2950.015.253345514
CCCGCCA1654.192716E-715.0026023
Produced by FastQC (version 0.11.5)