FastQCFastQC Report
Mon 6 Jun 2016
ERR1378254.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378254.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056471
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCG702.7031429E-522.49955212
CTTCGTA1305.7826946E-717.3073467
ATCTTCG1451.8078736E-615.5176665
TTCGTAT1056.037204E-414.9997018
TCGTATA1056.037204E-414.9997019
CGGCTAA1453.104472E-513.96523817
CGTATAA1301.926222E-413.84587810
CGCCACT1200.001656502113.1247399
CCGGCTA1555.8100166E-513.06425516
GCGATCT1250.00225164712.59974816
CACGCCC2401.7122329E-712.18725711
GCCCGGC2401.7122329E-712.18725714
ACCACGC2603.7034624E-812.1151439
TCGCTTG2054.8258316E-612.07292915
GTGGCGC1506.449291E-411.99976111
CGCCCGG1506.449291E-411.99976113
CCACGCC2706.258597E-811.66643310
CCCGGGT1558.492769E-411.61267145
AGTGGCG1953.8435894E-511.53823110
CACCACG3204.780304E-911.2497758
Produced by FastQC (version 0.11.5)