FastQCFastQC Report
Mon 6 Jun 2016
ERR1378250.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378250.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136112
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA403.458863E-739.37432
TCTCGTA401.5608222E-533.74914641
CTCGTAT453.4914156E-529.99923542
GCGGTTC453.4914156E-529.9992358
CGTATGC551.3684251E-424.5448344
CGGTAAA802.9753592E-622.4994334
GCGGTAA602.467909E-422.49942833
GACCGCG602.467909E-422.49942829
CCGAGAC602.467909E-422.49942825
CCGCGGT754.603475E-520.99946631
ACCGCGG654.238916E-420.76870330
TCCGAAC550.0039370920.45402532
ATCTCGT951.3160985E-518.94688640
ATCTTCG1507.2595867E-918.0011255
CTTCGTA1253.8341568E-717.9995447
AGCGGTT800.001711119116.8745737
TCGTATG800.001711119116.87457343
TTCGTAT1358.584466E-716.6662438
ATGCCGA952.8198663E-416.57852622
GAGCGGT900.003752323315.0009386
Produced by FastQC (version 0.11.5)