FastQCFastQC Report
Mon 6 Jun 2016
ERR1378249.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378249.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1301751
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA501.0822987E-940.50031332
GACCGGC651.4037141E-831.15408529
ACCGACA300.005148279529.9990819
ACCGGCG702.8865543E-828.92879530
GCGGTTC801.05394065E-725.3126958
CCGGCGT801.05394065E-725.31269531
GAGACCG851.8939136E-723.82371327
AGACCGG851.8939136E-723.82371328
GGCGTAA851.8946048E-723.82279833
TCTCGTA851.8946048E-723.82279841
GAGCGGT903.2873868E-722.5001746
ATCTCGT903.2886055E-722.4993140
TCGTATG903.2886055E-722.4993143
ATGCCGA903.2886055E-722.4993122
AATGCCG1056.1114406E-821.42791221
AGCGGTT955.5324927E-721.3159527
GCGTAAA955.53453E-721.31513434
CTCGTAT1009.059477E-720.24937842
CGGTTCA1009.059477E-720.2493789
GTTTGCG600.00651210418.74942439
Produced by FastQC (version 0.11.5)