FastQCFastQC Report
Mon 6 Jun 2016
ERR1378248.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378248.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413942
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAAA550.00393803620.45359823
CCTTCGT2550.018.5357631
ACCGCAA750.001110740517.99916616
CGCCCGG1651.2314558E-917.72645213
ACCACGC3050.016.9664259
ACGCCCG800.001711639116.87421812
CACGCCC2159.094947E-1216.74340811
CTTCGTT3150.016.433622
GCGCCCG1401.255803E-616.07068312
ACGCCCA1552.0681E-715.96700312
CCACGCC3100.015.96700310
CGTGAGC2651.8189894E-1215.2882571
CACCACG3300.014.9993058
TCGTTGG3250.014.5419024
CGTTGGA3250.014.53881555
CGGCTAA2102.9924195E-813.92792617
CGCCACC1951.8293031E-713.8464925
CGGGATT2306.67751E-913.69501814
TTCGTTG3650.013.5653323
ACGGGTT1000.007554464513.49937313
Produced by FastQC (version 0.11.5)