FastQCFastQC Report
Mon 6 Jun 2016
ERR1378241.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378241.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917731
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT50740.5528853226054258Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAATTAGATCTCGTATGCC32020.34890398166783076Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAATTAGATCTCGTATGCCGTC28780.313599518813247Illumina Paired End PCR Primer 2 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3650.038.8360945
TATGCCG3700.038.31127543
ATGCCGT3900.036.34659244
ATCGGAA6850.034.4857127
GATCGGA6950.033.98951326
AGATCGG7100.033.27142325
TCGGAAG8050.029.34498428
CGAGATC8250.029.17899123
GAGATCG8350.028.56010824
CCGAGAT8450.028.48836322
AATGCCG15650.024.7260418
ATGCCGA15650.024.58228319
TGCCGAG16000.023.90393420
GAATGCC16700.023.0366917
GCCGAGA17300.022.23773221
GGAATGC18200.021.13806316
ACGTGCC550.0039232620.4659462
CGGAAGA11750.020.10443529
AGGAATG19450.019.89524515
GTATGCC7300.019.41804542
Produced by FastQC (version 0.11.5)