FastQCFastQC Report
Mon 6 Jun 2016
ERR1378240.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378240.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060306
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT47810.4509075681925784Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAACTAAATCTCGTATGCC30830.29076511874873856Illumina Paired End PCR Primer 2 (97% over 37bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAACTAAATCTCGTATGCCGTC30150.2843518757792562Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG7500.035.3984825
GATCGGA7650.034.99849726
TATGCCG3450.034.56373243
ATCGGAA7900.034.17574727
TCGGAAG8700.032.0675928
GAGATCG8800.030.4248324
ATGCCGT3850.030.38830644
CGAGATC8850.030.2529423
TGCCGTC3900.029.99871345
CCGAGAT9200.029.1020122
ATGCCGA14300.027.84845519
AATGCCG14700.027.24372718
TGCCGAG14800.026.75560620
GCCGAGA15850.024.41535421
GAATGCC16600.024.39654217
CGGAAGA11700.023.84512929
GGAATGC17900.022.49903316
TGAACCG702.7036356E-522.49903310
GAAGAGC13800.020.37955931
CGAACTA6050.020.08178129
Produced by FastQC (version 0.11.5)