FastQCFastQC Report
Mon 6 Jun 2016
ERR1378239.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378239.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010408
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT50870.5034599884403133Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATACAAATCTCGTATGCCGTC35740.35371849787412607Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATACAAATCTCGTATGCC35630.3526298287424486Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3500.035.3569543
ATGCCGT3950.031.8985644
ATCGGAA7000.031.81967727
GATCGGA7100.031.37151526
AGATCGG7200.031.24828325
TCGGAAG7700.030.3879428
GTATGCG300.00514835229.99834811
ACGACTC300.00514835229.99834810
TGCCGTC4350.028.96535745
CCGAGAT7800.028.55612422
CGAGATC7850.028.37423723
GAGATCG8750.025.71287224
CCGATAC7700.024.54410431
ACCGATA7800.024.22943730
CGATACA7800.024.22943732
GACCGAT8050.023.47696929
AATGCCG15600.023.36410118
CGTATGC8150.023.19005844
ATGCCGA15900.023.06476819
TGCCGAG15750.022.99873520