FastQCFastQC Report
Mon 6 Jun 2016
ERR1378238.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378238.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766724
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT43230.5638274007335103Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAACAAATCTCGTATGCC29900.3899708369634966Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTAACAAATCTCGTATGCCGTC29440.3839712856255967Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3700.034.05854443
ATGCCGT3700.034.05854444
TGCCGTC3700.034.05854445
AGATCGG6000.033.3728725
GATCGGA6100.032.82577526
ATCGGAA6200.032.65920627
GTCGGAA300.005147807829.99808727
CGAGATC6850.029.56015823
TCGGAAG7200.029.37312728
CCGAGAT6900.029.34595722
GAGATCG6850.029.23171224
CACCGCG554.164627E-628.6345398
ACCGCGC608.190955E-626.2483279
CCGCGCC608.190955E-626.24832710
CGGGATT957.421477E-1026.05096814
ACGGGAT1159.094947E-1225.43315913
ATGCCGA14100.023.93464319
TGCCGAG14200.023.7660920
AATGCCG14300.023.59989418
GCCGAGA14400.022.65480421
Produced by FastQC (version 0.11.5)