FastQCFastQC Report
Mon 6 Jun 2016
ERR1378236.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378236.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635787
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT50670.7969650213043047Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAATAACATCTCGTATGCCGTC33560.527849735839204Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAATAACATCTCGTATGCC29900.4702832866982181Illumina Paired End PCR Primer 2 (97% over 34bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC6580.10349378014964132Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3650.042.53751843
ATGCCGT3850.039.15885544
TGCCGTC3850.039.15885545
GATCGGA6600.036.13345726
AGATCGG6650.035.8617825
ATCGGAA6650.035.5234627
TCGGAAG7050.033.8270728
CCGAGAT7900.030.18744522
CGAGATC7950.029.99758723
GAGATCG8000.029.810124
AATGCCG14400.027.3415518
ATGCCGA14600.026.81291219
TGCCGAG14600.026.81291220
GCCGAGA15300.025.4391321
GAATGCC15950.024.68453617
GGAATGC16750.023.37125616
CGGAAGA10350.023.04162629
ACCGCGC602.4671684E-422.4981929
AGGAATG17900.021.9954415
TCTCGTA6950.021.68890438
Produced by FastQC (version 0.11.5)