FastQCFastQC Report
Mon 6 Jun 2016
ERR1378235.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378235.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741131
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT41850.5646774996593046Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAACAATATCTCGTATGCC27930.3768564531776433Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAACAATATCTCGTATGCCGTC27880.37618180861413164Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4050.040.0025245
TATGCCG4000.039.94001443
ATGCCGT4300.037.6767944
GATCGGA5350.034.9040326
ATCGGAA5450.034.67640727
AGATCGG5500.033.95210325
TCGGAAG5900.032.7942528
CGAGATC6300.029.64072423
CCGAGAT6400.029.17758622
GAGATCG6600.028.29341924
ATGCCGA13550.026.06823319
AATGCCG13750.025.6890618
TGCCGAG13700.025.61859320
GCCGAGA14550.023.8127221
GAATGCC14950.023.4765717
GGAATGC15900.022.21538216
ACCGAAC7500.021.29846830
CGAACAA7550.021.15741732
CCGAACA7550.021.15741731
CTCGTAT7700.021.04028539
Produced by FastQC (version 0.11.5)