FastQCFastQC Report
Mon 6 Jun 2016
ERR1378234.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378234.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897449
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT43780.48782716343769955Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGTGATCTCGTATGCCGTC23360.2602933425743413Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGTGATCTCGTATGCC22370.24926207505941841Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3000.041.25107243
TGCCGTC3300.037.50097345
GATCGGA6300.036.0703626
ATGCCGT3500.036.0009444
AGATCGG6550.035.38062725
TCGGAAG6850.034.48802628
ATCGGAA6650.034.17191727
CCGAGAT7250.031.96456722
CGAGATC7300.031.74563223
GAGATCG7350.031.52967324
ATGCCGA12550.029.04296319
TGCCGAG12900.028.08056320
AATGCCG13000.027.86455518
GCCGAGA13350.026.62842221
GAATGCC14300.025.33141517
CGGAAGA9550.024.73748429
GGAATGC15050.024.36804616
CGAGTGT5700.021.7098829
CGTATGC5750.021.52229741
AGGAATG17200.021.3220415
Produced by FastQC (version 0.11.5)