FastQCFastQC Report
Mon 6 Jun 2016
ERR1378231.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378231.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634912
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT54070.8516140819515146Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGTGAATCTCGTATGCCGTC35100.5528325185222519Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGTGAATCTCGTATGCC32960.5191270601280177Illumina Paired End PCR Primer 2 (97% over 35bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC7110.11198402298271257Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4450.039.94549643
TGCCGTC4500.039.5016645
ATGCCGT4600.039.1320844
AGATCGG6300.038.9271425
GATCGGA6300.038.9271426
TCGGAAG6550.038.81536528
ATCGGAA6450.038.0218627
CCGAGAT6600.037.49862322
CGAGATC6800.036.0648523
GAGATCG7100.034.54098524
GGTCGGA300.00514646929.99889626
CGAGCTT300.00514646929.99889622
GTCGGAA300.00514646929.99889627
AATGCCG15300.028.52836218
ATGCCGA15400.028.34311519
TGCCGAG15400.028.19701620
GCCGAGA15350.027.5559921
GAATGCC16300.026.9161917
GGAATGC16650.026.35038416
AGGAATG18550.023.5301315
Produced by FastQC (version 0.11.5)