FastQCFastQC Report
Mon 6 Jun 2016
ERR1378230.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378230.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745074
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT35720.47941546745692376Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGTGAATCTCGTATGCC19530.26212161476578166Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGTGAATCTCGTATGCCGTC19220.2579609542139438Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG5300.039.05473725
TCGGAAG5550.038.1062928
GATCGGA5450.037.9798426
ATCGGAA5500.037.63456727
TATGCCG3100.034.83938243
ATGCCGT3250.033.23141544
TGCCGTC3300.032.7279145
CGAGATC6350.032.5968723
CCGAGAT6400.032.34220522
GAGATCG6400.032.34220524
GTTCGTA300.0051473129.9985711
ATGCCGA11400.028.41969719
AATGCCG11450.028.29559318
TGCCGAG11550.028.0506120
GAATGCC12350.026.41574317
CGGAAGA8150.025.9496829
GCCGAGA12300.025.79145221
GGAATGC13200.024.54428316
AGCGGTT15550.021.4180814
GAGCGGT15400.021.337313
Produced by FastQC (version 0.11.5)