FastQCFastQC Report
Mon 6 Jun 2016
ERR1378229.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378229.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873645
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT50800.5814718793102461Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGCATGAATCTCGTATGCCGTC26960.30859216272055584Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCATGAATCTCGTATGCC24960.2856995690469241Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4250.037.05726643
ATGCCGT4250.037.05726644
TGCCGTC4350.036.20537645
GATCGGA6300.034.28427526
AGATCGG6400.033.7485825
ATCGGAA6350.033.66000427
TCGGAAG6850.032.18842728
CGAGATC6650.032.14150623
GAGATCG6800.031.43250724
CCGAGAT6950.030.75410722
AATGCCG14100.026.80738618
ATGCCGA14150.026.7126619
TGCCGAG14400.025.9364120
GCCGAGA15150.024.5039221
GAATGCC16450.023.1145317
ATGCCCG702.70291E-522.49905612
GGAATGC17250.022.17298316
CGGAAGA10300.021.1884329
AGGAATG18350.020.96642115
CGTATGC7550.020.86005241
Produced by FastQC (version 0.11.5)