FastQCFastQC Report
Mon 6 Jun 2016
ERR1378228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691030
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT44220.6399143307815869Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCGAATCTCGTATGCCGTC27850.4030215764872726Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCGAATCTCGTATGCC23220.3360201438432485Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3700.038.91723343
ATGCCGT3850.037.40097444
TGCCGTC3850.037.40097445
AGATCGG6050.034.2133925
GATCGGA6100.033.93295726
ATCGGAA6200.033.38564727
TCGGAAG6600.032.72585328
CCGAGAT6950.030.10660722
GAGATCG6950.029.7828824
CGAGATC7050.029.67956423
AATGCCG12650.028.99083518
ACGGGTT554.163183E-628.6351213
ATGCCGA12850.028.53961819
TGCCGAG13000.028.21031220
GCCGAGA13000.027.69110321
GAATGCC14100.026.32864417
ACCGGAT5700.025.26206227
CCGGATC5700.025.26206228
CGAATCT5700.025.26206234
TCGAATC5750.025.0423933
Produced by FastQC (version 0.11.5)