FastQCFastQC Report
Mon 6 Jun 2016
ERR1378227.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378227.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905039
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT59080.6527895482957088Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGACGTATCTCGTATGCCGTC37220.41125299572725593Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGACGTATCTCGTATGCC29230.3229695073913942Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5000.040.05122843
TGCCGTC5200.038.510845
ATGCCGT5400.037.0844744
GATCGGA7750.033.3862826
ATCGGAA8100.032.2214327
AGATCGG8050.032.14206725
TCGGAAG8400.031.60636728
CGGCGGA300.00514730529.99926438
CGAGATC8850.029.74503123
GAGATCG8800.029.65836124
CCGAGAT9300.028.30575822
ATGCCGA17450.026.94776319
AATGCCG17700.026.6942618
TGCCGAG17650.026.5149320
GAATGCC18900.024.88034217
GCCGAGA18800.024.6536521
CGTATCT7950.024.33902534
ACGTATC8050.024.31617233
CGTATGC8250.024.27347441
CGAGACG8450.024.23017529
Produced by FastQC (version 0.11.5)