FastQCFastQC Report
Mon 6 Jun 2016
ERR1378226.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378226.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696340
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT38290.5498750610334032Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCGTAGAATCTCGTATGCCGTC20420.29324755148347076Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGTAGAATCTCGTATGCC16260.23350662032914954Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA5050.035.19717827
TATGCCG3100.034.83787543
AGATCGG5200.034.6145625
GATCGGA5250.034.28489326
TCGGAAG5600.032.9456428
ATGCCGT3450.031.95575744
TGCCGTC3450.031.95575745
CCGAGAT6000.030.74926222
AATGCCG10400.028.98969318
TGCCGAG10450.028.85098520
GAGATCG6250.028.79930924
CGAGATC6300.028.57074523
ATGCCGA10600.028.44271519
CGCGTAG4150.026.56562829
GCCGAGA11250.026.39936821
GAATGCC11400.026.24937217
CCGCGTA4250.025.94055728
GGAATGC11500.025.82546816
ACCGCGT4450.024.77468727
TCGTATG4450.024.77468740
Produced by FastQC (version 0.11.5)