FastQCFastQC Report
Mon 6 Jun 2016
ERR1378225.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378225.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864905
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT39200.4532289673432342Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGAGAATCTCGTATGCCGTC29050.3358750382990039Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGAGAATCTCGTATGCC24080.2784120799394153Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3800.037.89337543
TGCCGTC4200.034.82017545
ATGCCGT4250.033.3517444
GATCGGA6100.031.72017126
ATCGGAA6150.031.09644327
TCGGAAG6350.030.47134428
AGATCGG6400.030.23328825
GAGATCG6700.029.88698424
CCGAGAT7000.027.9632822
AATGCCG12500.026.81903518
CGAGATC7500.026.39905223
ATGCCGA12800.026.19046419
TGCCGAG12900.025.98743620
CGAGAAT5850.025.38370332
TCTCGTA5700.025.2622538
GCCGAGA13450.025.25931521
TCCCGTA450.001226566424.999124
CGTATGC5900.024.40590341
CTCGTAT5900.024.40590339
ACCGTCG5950.024.2008127
Produced by FastQC (version 0.11.5)