FastQCFastQC Report
Mon 6 Jun 2016
ERR1378224.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378224.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947191
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT40870.43148636336282753Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGAGTATCTCGTATGCCGTC24390.2574982236951154Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGAGTATCTCGTATGCC21330.22519217348982412Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3100.037.7403443
ATGCCGT3450.033.91161344
GATCGGA5550.032.8364526
TGCCGTC3650.032.6698545
ATCGGAA5650.031.8570627
TCGGAAG5850.031.1525328
AGATCGG6050.030.12269425
TCGTCAC406.155443E-428.1327252
GAGATCG6700.027.20034424
CCGAGAT7100.025.9848222
CGAGATC7050.025.84997223
TGCCGAG11750.025.6584920
ATGCCGA11800.025.5497719
AATGCCG11900.025.33506618
GCCGAGA13100.022.84254621
GAATGCC13550.022.58207117
AGACCGC5400.021.6657525
GACCGCA5450.021.46698426
CGCAGAG5550.021.0801929
GAGACCG5700.020.9201724
Produced by FastQC (version 0.11.5)