FastQCFastQC Report
Mon 6 Jun 2016
ERR1378223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753667
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT39920.5296768997448476Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCAGAATCTCGTATGCC22080.29296758382680943Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCAGAATCTCGTATGCCGTC20990.2785049630672432Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT2900.038.79464744
TATGCCG3000.038.25152643
TGCCGTC3050.036.8867145
ATCGGAA6650.035.8637127
AGATCGG6750.035.66571825
TCGGAAG6950.035.28683528
GATCGGA6850.035.1450526
CGAGATC7850.030.95459223
GAGATCG7900.030.75867824
CCGAGAT8050.030.18553422
AATGCCG13100.027.82368918
ATGCCGA13100.027.65193719
TGCCGAG13200.027.27200120
CGGAAGA9450.026.18978129
GAATGCC14200.025.66833317
GCCGAGA13850.025.34228921
GGAATGC15150.024.05876516
CGTATGC5150.022.2824441
GAAGAGC11300.021.90207331
CTCGTAT5350.021.8700339
Produced by FastQC (version 0.11.5)