FastQCFastQC Report
Mon 6 Jun 2016
ERR1378222.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378222.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1489947
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT65800.44162644711523297Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCAGTATCTCGTATGCCGTC32470.21792721486066283Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCAGTATCTCGTATGCC26070.1749726668129806Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3900.036.9215143
AGATCGG8350.035.5673525
GATCGGA8350.035.297926
ATGCCGT4200.034.8199544
ATCGGAA8500.034.41030527
TGCCGTC4250.034.41030545
TCGGAAG8850.033.81212228
CGAGATC9500.030.78816623
GAGATCG9900.029.54419924
CCGAGAT10000.029.24875822
AATGCCG15800.028.62220218
ATGCCGA15850.028.5319119
TGCCGAG16100.027.6696320
GCCGAGA17150.025.45081321
CGGAAGA12000.025.31142429
GAATGCC18450.024.51115217
CTCGTAT6550.022.32729539
GGAATGC20250.022.22127716
CGTATGC6600.021.81725341
TCTCGTA6700.021.49162538
Produced by FastQC (version 0.11.5)