FastQCFastQC Report
Mon 6 Jun 2016
ERR1378221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918068
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT40530.44147056645041544Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCTCTATCTCGTATGCCGTC24670.2687164785179311Illumina Paired End PCR Primer 2 (97% over 35bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCTCTATCTCGTATGCC15990.17417010504668498Illumina Paired End PCR Primer 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3250.037.383343
GATCGGA5550.036.07981526
ATGCCGT3350.035.59576844
TGCCGTC3450.034.56400745
ATCGGAA5800.034.13673427
AGATCGG5950.034.0324225
TCGGAAG6450.032.09189628
CGAGATC6750.029.66562723
ATGCCGA11050.029.52384819
AATGCCG11100.029.3908618
CCGAGAT6900.029.34679822
GAGATCG6850.029.2325524
TGCCGAG11650.028.0033120
GCCGAGA11950.026.73546421
GACCGCT4900.025.25421526
GTCGGAA450.001226617724.99912327
GAATGCC13150.024.6379217
CGGAAGA8350.024.52009629
CTCGTAT5050.024.05856139
AGACCGC5050.024.05856125
Produced by FastQC (version 0.11.5)