FastQCFastQC Report
Mon 6 Jun 2016
ERR1378219.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378219.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681674
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT39050.5728544729592151Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGATACGATCTCGTATGCCGTC21750.3190674721347741Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATACGATCTCGTATGCC14090.20669704286799848Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT2850.037.89282244
TCGGAAG5000.037.79808828
AGATCGG4950.037.27084425
TATGCCG2900.037.239543
ATCGGAA4900.037.19227
GATCGGA5050.036.53280626
TGCCGTC2900.036.46367645
GAGATCG5300.034.80956324
CGAGATC5450.034.26432423
CCGAGAT5750.033.2591922
AATGCCG9750.030.92151518
TGCCGAG9800.030.76375420
ATGCCGA9900.030.45300719
GCCGAGA10150.029.25960421
GAATGCC10900.027.65915317
CGGAAGA6900.027.38992129
GGAATGC11400.026.6433916
CCGGATA4300.025.11501128
ACCGGAT4400.024.54421227
CTCGTAT4400.024.54421239