FastQCFastQC Report
Mon 6 Jun 2016
ERR1378218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences809039
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT45890.5672161663405596Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGAGTACATCTCGTATGCCGTC26670.3296503629614889Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGAGTACATCTCGTATGCC22680.2803325921247307Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA6200.038.82888427
GATCGGA6350.037.9116726
TATGCCG3350.037.6125643
TCGGAAG6550.037.4410528
AGATCGG6550.037.09755725
ATGCCGT3500.036.0005944
TGCCGTC3500.036.0005945
AACGGGT250.002107209335.99836712
CCGAGAT7100.034.223822
CGAGATC7400.032.8363523
GAGATCG7700.031.84920524
TGCCGAG12500.029.69865220
AATGCCG12700.029.40811718
ATGCCGA12700.029.40811719
GCCGAGA13100.027.99491521
CGGAAGA9100.026.9493329
GAATGCC14200.026.30162417
GGAATGC14750.025.16835216
GACCGGA5700.022.10426126
CTCGTAT5750.021.91340439
Produced by FastQC (version 0.11.5)