FastQCFastQC Report
Mon 6 Jun 2016
ERR1378217.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378217.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837703
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT41260.49253733124985827Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGTAGATCTCGTATGCC20350.2429261922184832Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGTAGATCTCGTATGCCGTC19530.23313752009960573Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG6550.036.75431425
TATGCCG2700.036.66525743
GATCGGA6600.036.4758726
ATCGGAA6700.035.93145427
TCGGAAG7100.035.174728
ATGCCGT3000.033.74870344
GAGATCG7150.033.67003324
CGAGATC7550.032.18419323
CCGAGAT7800.031.15264922
TGCCGTC3300.029.99884645
TGCCGAG11800.029.36327720
AATGCCG11850.029.23938218
ATGCCGA11850.029.23938219
CGGAAGA8900.028.56631729
GCCGAGA12350.027.50906421
GAATGCC13700.025.61945217
GGAATGC14550.024.12278416
GAAGAGC10850.023.01754831
GACCGCA4500.022.49913626
ACCGCAG4600.022.01002327
Produced by FastQC (version 0.11.5)