FastQCFastQC Report
Mon 6 Jun 2016
ERR1378216.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378216.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119853
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT70440.6290111291392709Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTGAGATCTCGTATGCCGTC36310.3242389849382017Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTGAGATCTCGTATGCC26960.24074588361150973Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4900.038.57112543
ATGCCGT4950.037.72697444
TGCCGTC4950.037.2724345
AGATCGG9200.035.70464725
GATCGGA9300.035.32072426
ATCGGAA9200.035.2155427
TCGGAAG9950.033.46557628
CCGAGAT10900.030.3424422
CGAGATC11100.029.7957323
GAGATCG11100.029.7957324
AATGCCG17850.029.6202918
TGCCGAG17800.029.57709720
ATGCCGA18200.028.8034319
GAATGCC19750.026.77074417
GCCGAGA19500.026.65244721
CGGAAGA12900.025.63818729
TCTCGTA7600.024.86711338
CTCGTAT7650.024.70568739
GACCGTG7650.024.70458226
GAGACCG7700.024.54416524
Produced by FastQC (version 0.11.5)