FastQCFastQC Report
Mon 6 Jun 2016
ERR1378215.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378215.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071345
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT64350.6006468504543354Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGACATCTCGTATGCCGTC34990.32659880804036046Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGACATCTCGTATGCC30850.2879557938852564Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4600.038.15201643
TGCCGTC4600.037.66288845
ATGCCGT4900.035.35699544
GATCGGA7950.034.8095526
AGATCGG8050.034.37713225
ATCGGAA8200.033.74828327
TCGGAAG8950.032.4285228
GAGATCG9400.029.43999524
CCGAGAT9450.029.28422522
CGAGATC10000.027.8985823
AATGCCG17750.026.36485718
ATGCCGA17850.026.21715519
TGCCGAG18250.025.5192520
GAATGCC18800.024.89235317
GCCGAGA18800.023.93495421
CGGAAGA12500.023.2188229
GGAATGC20450.022.99394216
GGTCGGA500.002265179322.49885626
GTCGGAA500.002265179322.49885627
CGAGTGA8200.021.40135229
Produced by FastQC (version 0.11.5)