FastQCFastQC Report
Mon 6 Jun 2016
ERR1378214.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378214.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742737
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT63590.8561576978122808Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGAGACATCTCGTATGCCGTC43530.5860755556812169Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGAGACATCTCGTATGCC29320.3947561519084144Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC9130.1229237267027225Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4450.036.90867644
TATGCCG4600.036.68335743
TGCCGTC4700.034.9454545
GATCGGA8850.031.77842526
ATCGGAA9000.030.99879527
AGATCGG9150.030.7365125
TCGGAAG9450.030.2369228
CCGAGAT9400.030.15840122
CGAGATC9450.029.76074823
GAGATCG10150.027.92994724
ATGCCGA18550.024.50039519
AATGCCG18650.024.36902618
TGCCGAG18600.024.31356820
GCCGAGA18850.023.63303421
GAATGCC19650.023.12887217
GGAATGC20750.022.01119216
CGGAAGA13200.021.64688529
AGGAATG21550.021.29847715
CAGCGGA550.003936132420.4537511
CTCGTAT8450.019.96963739
Produced by FastQC (version 0.11.5)