FastQCFastQC Report
Mon 6 Jun 2016
ERR1378213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693457
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT50100.7224672906899778Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGAGCATATCTCGTATGCCGTC32450.4679453808960036Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGAGCATATCTCGTATGCC25350.3655597967862463Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC7280.10498127497451176Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT3750.038.4009244
TATGCCG3800.037.8956543
AGATCGG7150.037.7604225
TCGGAAG7200.037.49819628
GATCGGA7150.037.4457526
ATCGGAA7150.037.1310827
TGCCGTC4200.034.2865445
CGAGATC7900.034.1755723
CCGAGAT7950.033.96063222
GAGATCG8150.033.1272424
AACGGGT352.8200267E-432.1413112
ACGGGTT300.005147077629.99855613
AATGCCG14200.029.3119718
ATGCCGA14350.029.00557519
TGCCGAG14450.028.80484220
GCCGAGA14700.028.0088621
GAATGCC15750.026.85585217
GGAATGC16200.025.97097216
CGGAAGA10600.025.68272829
AGGAATG17750.024.0833515
Produced by FastQC (version 0.11.5)