FastQCFastQC Report
Mon 6 Jun 2016
ERR1378212.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378212.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423425
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT67091.584460057861487Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGACACATCTCGTATGCCGTC41140.9716006376572002Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGACACATCTCGTATGCC33420.7892779122630927Illumina Paired End PCR Primer 2 (97% over 34bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC9480.2238885280746295Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4750.042.15767344
TATGCCG4850.041.7523643
TGCCGTC5000.040.94978745
AGATCGG9150.037.62275325
CCGAGAT9350.037.29926722
GATCGGA9250.037.21602226
TCGGAAG9800.036.96409228
GAGATCG9400.036.62215424
ATCGGAA9450.036.42838327
CGAGATC9650.036.37286823
CGTTACA250.00210566335.99981344
GAACCGC250.00210566335.99981311
ACCGCTC352.8174752E-432.1426913
AATGCCG18950.030.3956218
TGCCGAG19050.030.11795220
ATGCCGA19150.030.0781719
TCTTCCG300.005143761629.99984418
AGGTCGG602.4129804E-729.99984425
GCCGAGA18900.029.28556321
GAATGCC20000.028.7998517
Produced by FastQC (version 0.11.5)