FastQCFastQC Report
Mon 6 Jun 2016
ERR1378211.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378211.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671248
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT50590.7536707744380616Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTCTAATCTCGTATGCCGTC29160.43441470216671035Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTCTAATCTCGTATGCC25890.38569947322003195Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC7650.11396682001287153Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT3800.037.89373844
TATGCCG4100.035.66978543
TGCCGTC4050.035.55461545
GATCGGA6800.033.74910726
GGTCGGA401.5598862E-533.74910426
TCGGAAG7300.033.28679328
AGATCGG6900.033.25998725
ATCGGAA6900.033.25998727
CCGAGAT7950.030.28221922
CGAGATC8250.028.90832523
GAGATCG8050.028.78805724
TGCCGAG14600.027.27667620
AATGCCG14700.027.24417718
ATGCCGA15100.026.52247819
GCCGAGA15050.025.56410821
GAATGCC15850.025.26746717
GGAATGC15950.025.25011416
CGGAAGA10050.024.40233829
AGGAATG17500.023.14224415
GTCGGAA602.466468E-422.49940327
Produced by FastQC (version 0.11.5)