FastQCFastQC Report
Mon 6 Jun 2016
ERR1378210.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378210.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572837
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT47000.8204777275210924Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTATCTGATCTCGTATGCCGTC28130.4910646484078368Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTATCTGATCTCGTATGCC20520.35821708444112377Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC6280.10962978997515872Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3750.038.39940643
ATGCCGT3950.037.02474244
TGCCGTC4050.036.11055445
AGATCGG6400.033.74947725
GATCGGA6500.033.576426
ATCGGAA6600.033.0676727
TCGGAAG6900.031.62994428
GCGAAAG300.005143323430.0021516
CGAGATC7450.029.89886323
GAGATCG7400.029.49278524
AATGCCG13050.028.27542118
CCGAGAT7900.028.19576522
ATGCCGA13250.027.84862319
GAATGCC13550.027.398117
TGCCGAG13500.027.3329120
CCGTATC5550.025.94554528
GCCGAGA14400.025.46835521
CGTATCT5700.025.26276629
GACCGTA5700.025.26276626
AGACCGT5750.025.0430925
Produced by FastQC (version 0.11.5)