FastQCFastQC Report
Mon 6 Jun 2016
ERR1378209.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378209.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565844
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT43160.7627543987388751Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATACTCATCTCGTATGCCGTC24690.43633934441294775Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATACTCATCTCGTATGCC14840.2622630972494186Illumina Paired End PCR Primer 2 (97% over 35bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC5730.10126465951746419Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG5500.037.63451825
ATCGGAA5550.037.29546727
GATCGGA5600.036.9624726
TCGGAAG5750.036.3895228
TATGCCG2950.035.84886643
TGCCGTC3100.034.8400845
ATGCCGT3250.033.23207544
GAGATCG6250.033.11837424
CCGAGAT6350.032.59682522
AATGCCG9750.032.53686518
CGAGATC6400.032.3421623
ATGCCGA10200.031.32199119
TGCCGAG10200.031.32199120
GAATGCC10450.030.3573617
CGGAAGA6950.029.78271129
GGAATGC10700.029.64807516
GCCGAGA10850.029.0308321
CCGATAC3750.028.79858628
CGATACT3800.028.41965929
ACCGATA3850.028.05057127