FastQCFastQC Report
Mon 6 Jun 2016
ERR1378207.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378207.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912310
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT53570.5871907575275948Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTATGATCTCGTATGCCGTC31440.3446197016365051Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTATGATCTCGTATGCC20780.22777345419868247Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3500.038.57086643
ATGCCGT3900.035.19179544
TGCCGTC3950.034.17671645
TCGGAAG7150.033.67083728
GATCGGA7050.033.19100626
ATCGGAA7150.033.04147327
AGATCGG7100.032.95726425
CCGAGAT8600.028.25540222
ATGCCGA14100.027.4464119
TGCCGAG14000.027.3210320
AATGCCG14250.026.99960518
CGAGATC9100.026.45565623
GAGATCG9200.025.67897224
GAATGCC15200.025.3121317
GCCGAGA15450.024.46566221
GGAATGC16550.023.65524316
CGGAAGA10300.023.37344629
AGGAATG17900.021.8711915
CGTCTAT6300.021.42825929
CCGTCTA6300.021.42825928
Produced by FastQC (version 0.11.5)