FastQCFastQC Report
Mon 6 Jun 2016
ERR1378206.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378206.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808654
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT67840.8389249295743297Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTAGTATCTCGTATGCCGTC38520.47634711508259403Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTAGTATCTCGTATGCC36380.4498833864668944Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC9380.11599522168937518Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5200.040.2432643
TGCCGTC5150.040.1970545
ATGCCGT5300.039.05939544
AGATCGG7900.036.45379325
GATCGGA7950.036.2245226
ATCGGAA7950.036.2245227
CGCCCGG458.699244E-734.99817313
TCGGAAG8500.034.4099728
CCGAGAT8550.034.2087422
GAGATCG8750.033.16969724
CGAGATC8900.032.61065723
AATGCCG18100.027.71954718
ATGCCGA18100.027.71954719
TGCCGAG18150.027.64318520
GCCGAGA18950.026.4761921
GAATGCC19400.026.32594717
GGAATGC20950.024.16341416
AGGAATG21100.023.88500615
CGGAAGA12250.023.87630729
CGGCTAA851.8931496E-723.82228717
Produced by FastQC (version 0.11.5)