FastQCFastQC Report
Mon 6 Jun 2016
ERR1378205.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378205.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770555
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT44060.5717956537820142Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGTCTATCTCGTATGCCGTC25070.3253499101297117Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGTCTATCTCGTATGCC19810.25708742399958473Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA6450.038.0217826
ATCGGAA6550.037.4412927
TCGGAAG6700.036.9388628
AGATCGG6700.036.60305425
TATGCCG2800.036.1616543
ATGCCGT2800.035.3580644
CCGAGAT7100.034.857822
GAGATCG7350.033.9782724
CGAGATC7400.033.44464523
TGCCGTC2950.032.7974645
ATGCCGA12300.028.71839519
AATGCCG12350.028.60212318
TGCCGAG12600.028.21318820
GCCGAGA12700.027.4595621
GAATGCC13200.026.41942817
CGGAAGA9500.026.05161929
GGAATGC14800.023.71529216
CGTAGTC5250.022.71340232
CCGTAGT5300.022.49912331
TCGTATG5450.022.29415543
Produced by FastQC (version 0.11.5)