FastQCFastQC Report
Mon 6 Jun 2016
ERR1378204.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378204.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810931
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT53870.6642981955308158Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTATCTATCTCGTATGCCGTC30060.3706850521191075Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTATCTATCTCGTATGCC23730.2926266229802536Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4100.037.32060244
TATGCCG4050.037.22574243
TGCCGTC4200.036.43201445
ACGATAA250.002107041235.99896210
GATCGGA7150.034.29971326
TCGGAAG7250.034.13694828
ATCGGAA7150.033.98503527
AGATCGG7300.033.90313325
GGACCGA352.8201265E-432.141937
CGAGATC8000.031.49909423
CCGAGAT8350.030.17877222
GAGATCG8400.029.73128724
AATGCCG14350.028.37897518
ATGCCGA14500.028.08539819
TGCCGAG14800.027.516120
GAATGCC15650.026.1653817
GCCGAGA15700.025.79543721
GGAATGC16400.024.96879416
CGGTATC6250.024.83928529
CGGAAGA10050.024.62615829
Produced by FastQC (version 0.11.5)