FastQCFastQC Report
Mon 6 Jun 2016
ERR1378203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540596
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT79811.4763335281800087Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATCAATCTCGTATGCCGTC46050.85183760146209Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATCAATCTCGTATGCC30320.5608624555120645Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC10210.18886562238714308Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGGA451.9254003E-839.9993426
ATGCCGT5450.039.63237844
TGCCGTC5450.039.63237845
TATGCCG5400.039.58268443
GATCGGA10850.038.15605526
ATCGGAA10800.037.91604227
TCGGAAG11300.037.63212628
AGATCGG11050.037.46544625
GAGATCG11300.037.0347924
CGAGATC11550.036.62277623
CCGAGAT11650.036.5015522
GTCGGAA551.1259908E-732.72673427
AATGCCG21150.031.0633218
ATGCCGA21200.030.99005519
TGCCGAG21250.030.81125620
GCCGAGA21400.029.9644621
GAATGCC22200.029.39140917
GGAATGC22750.028.77974516
AGGAATG23200.028.2215215
CGGAAGA15100.028.16178729
Produced by FastQC (version 0.11.5)