FastQCFastQC Report
Mon 6 Jun 2016
ERR1378202.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378202.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599880
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT62441.0408748416349936Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTGTATATCTCGTATGCC39720.662132426485297Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTGTATATCTCGTATGCCGTC37160.619457224778289Illumina Paired End PCR Primer 2 (96% over 32bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC8940.14902980596119222Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4800.041.25137744
TATGCCG4800.040.7826143
TGCCGTC4950.040.0046745
ATCGGAA7750.033.67573527
GATCGGA7850.033.24674226
AGATCGG7750.033.0951225
CCGAGAT7850.032.96013322
TCGGAAG8400.032.1412528
CGAGATC8000.032.06089823
GAGATCG8300.030.63099724
AGCGCAT406.164482E-428.12359435
AATGCCG17500.027.12721418
ATGCCGA17450.026.94707519
TGCCGAG17850.026.3432220
GCCGAGA17850.025.58695621
GAATGCC18800.025.37107317
GGAATGC18950.025.0515216
GGTCGGA450.001226228924.99874926
GTCGGAA450.001226228924.99874927
AGGAATG20100.023.84209315
Produced by FastQC (version 0.11.5)