FastQCFastQC Report
Mon 6 Jun 2016
ERR1378200.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378200.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503729
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT67181.333653611366429Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCATCATATCTCGTATGCCGTC34600.6868772693253713Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCATCATATCTCGTATGCC31480.624939203420887Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC9330.1852186393874484Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT3850.038.5700744
TATGCCG3950.037.5936143
TGCCGTC4000.037.12368845
ATCGGAA8750.033.4273927
AGATCGG8900.033.11680625
GATCGGA9000.032.99883726
TCGGAAG9000.032.99883728
GAGATCG9000.032.74884424
CCGAGAT9150.032.2119822
CGAGATC9450.031.18937723
ACGCCCA406.162569E-428.12400612
TGCCGAG16650.027.16120320
AATGCCG16900.027.15880418
ATGCCGA16900.026.89254219
GCCGAGA17150.025.9757621
GAATGCC17750.025.73148517
CCGTCCA450.001225850124.99911940
GGAATGC18300.024.95813616
CGGAAGA12050.024.45971929
AGGAATG19350.023.72009315
Produced by FastQC (version 0.11.5)