FastQCFastQC Report
Mon 6 Jun 2016
ERR1378199.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378199.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716354
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT84481.1793052038517269Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCACATATCTCGTATGCCGTC42660.5955156249563763Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCACATATCTCGTATGCC41810.5836499831089099Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC11430.1595579839018139Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4750.041.20784444
TATGCCG5050.038.75985343
TGCCGTC5100.038.37985645
AGCACGT250.00210725335.99765830
GATCGGA11250.033.797826
AGATCGG11400.033.3530925
ATCGGAA11200.033.34604327
TCGGAAG11750.032.93402528
CCGAGAT11750.032.93402522
CGAGATC11850.032.4662423
GAGATCG11850.032.4662424
GGTCGGA501.981247E-631.4979526
GTCGGAA501.981247E-631.4979527
ATGCCGA22400.026.91788919
AATGCCG22650.026.6207818
TGCCGAG22800.026.34696620
GCCGAGA22550.025.84088921
GAATGCC23950.025.17581417
AACGGGT450.001226569424.99837112
GGAATGC24500.024.79430216