FastQCFastQC Report
Mon 6 Jun 2016
ERR1378197.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378197.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286748
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT71052.4777853725222143Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTATAATCTCGTATGCC44171.540376916316766Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTATAATCTCGTATGCCGTC43731.525032432658641Illumina Paired End PCR Primer 2 (96% over 31bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC10140.3536206006667876Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGGA253.8850587E-544.9968626
GTCGGAA302.1608212E-644.9968627
ATGCCGT5600.042.9955744
TATGCCG5800.041.90093643
TGCCGTC5850.041.15815445
AGATCGG8950.034.6903225
GATCGGA8950.034.6903226
ATCGGAA8900.034.63241627
GAGATCG9050.034.555624
CGAGATC9200.034.2367423
TCGGAAG9350.033.92811228
CCGAGAT9400.033.508322
CGGTGGA300.00514264529.9979069
AATGCCG19900.028.49047518
AGGTCGG406.1572826E-428.12303525
ATGCCGA20200.028.06734819
GAATGCC20250.027.99804517
TGCCGAG20250.027.99804520
GCCGAGA20150.027.5787221
GGAATGC20600.027.5223516
Produced by FastQC (version 0.11.5)