FastQCFastQC Report
Mon 6 Jun 2016
ERR1378195.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378195.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522127
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAATACAATCTCGTATGCC12530.23997992825500308Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1700.041.02574541
CGTATGC1650.040.90543444
CTCGTAT1700.039.7023342
ATCTCGT1750.038.56797840
CGAATAC1750.038.56797832
TCGTATG1750.038.56797843
GACCGAA1850.036.48322329
ACCGAAT1950.034.61229330
GCGGTTC2150.033.485388
GAGCGGT2050.032.9428146
AGAGCGG2050.032.9428145
CGGTTCA2050.032.9238859
AATCTCG2050.032.92388539
CGGAAGA2000.032.659621
AGACCGA2100.032.13998428
CCGAGAC2100.032.13998425
CCGAATA2100.032.13998431
GAGACCG2150.031.39254227
AGCGGTT2400.030.9347327
AAGAGCG2200.030.6967124
Produced by FastQC (version 0.11.5)